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cheA [2017-10-08 15:40:51]
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cheA [2017-10-08 15:40:51]

two-component sensor kinase, chemotactic signal modulator
Locus
BSU16430
Isoelectric point
4.45
Molecular weight
74.59 kDa
Protein length
671 aa Sequence Blast
Gene length
2013 bp Sequence Blast
Function
chemotactic signal modulator
Product
two-component sensor kinase
Essential
no
Synonyms
cheN
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Information processing → Protein synthesis, modification and degradation → Protein modification → Protein kinases
  • Information processing → Regulation of gene expression → Transcription factors and their control → Control of two-component response regulators → Two-component sensor kinase
  • Lifestyles → Exponential and early post-exponential lifestyles → Motility and chemotaxis → Signal transduction in motility and chemotaxis → Soluble signalling proteins
  • Lifestyles → Exponential and early post-exponential lifestyles → Biofilm formation → Other proteins required for efficient pellicle biofilm formation
  • Lifestyles → Exponential and early post-exponential lifestyles → Swarming
  • Groups of genes → Membrane proteins
  • Groups of genes → Phosphoproteins → Phosphorylation on a His residue

    • Gene

    Coordinates
    1,712,815 → 1,714,833

    Phenotypes of a mutant

  • not essential for pellicle biofilm formation, but mutant is outcompeted by the wild-type strain when competed during pellicle formation PubMed

    • The protein

    Catalyzed reaction/ biological activity

  • autophosphorylation, phosphorylation of CheY and CheB PubMed

    • Modification

  • autophosphorylation on a His residue

    • Effectors of protein activity

  • phosphorylation of CheA is stimulated by interaction with the complex of deaminated McpC-CheD PubMed
  • the kinase activity of CheA is activated by chemoreceptors bound to CheD PubMed

    • Localization

  • membrane (according to Swiss-Prot)
  • localizes to the cell poles PubMed

    • Expression and Regulation

    Operons

    Genes
    ylxF-fliK-flgD-flgE-swrD-fliL-fliM-fliY-cheY-fliZ-fliP-fliQ-fliR-flhB-flhA-flhF-flhG-cheB-cheA-cheW-cheC-cheD-sigD-swrB
    Description
    PubMed

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • SigD: sigma factor, PubMed, in SigD regulon

    • Regulatory mechanism

  • Spo0A: repression, in Spo0A regulon
  • CodY: repression, in CodY regulon
  • DegU: regulation, in DegU regulon
  • SwrA: activation, in SwrA regulon

    • Regulation

  • see fla-che operon
    • view in new tab

    Genes
    flgB-flgC-fliE-fliF-fliG-fliH-fliI-fliJ-ylxF-fliK-flgD-flgE-swrD-fliL-fliM-fliY-cheY-fliZ-fliP-fliQ-fliR-flhB-flhA-flhF-flhG-cheB-cheA-cheW-cheC-cheD-sigD-swrB
    Description
    PubMed

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • SigD: sigma factor, PubMed, in SigD regulon

    • Regulatory mechanism

  • Spo0A: repression, in Spo0A regulon
  • SwrA: activation, in SwrA regulon
  • CodY: repression, in CodY regulon
  • DegU: repression, in DegU regulon

    • Regulation

  • expression during spore germination is strongly reduced under conditions of osmotic stress PubMed
    • view in new tab

    Biological materials

    Mutant

  • 1A797 ( cheA::cat), PubMed, available at BGSC
  • DS6887 (marker-less in NCIB3610) PubMed
  • BKE16430 (ΔcheA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_ATACTGATTCATATCCATTT, downstream forward: _UP4_TAACCATTCGAGGAGGTGTC
  • BKK16430 (ΔcheA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_ATACTGATTCATATCCATTT, downstream forward: _UP4_TAACCATTCGAGGAGGTGTC

    • Labs working on this gene/protein

  • Daniel Kearns

    • References

    Reviews

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    Original publications

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